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サッカー 近藤 昭彦

Biotinylated bionanocapsules for displaying diverse ligands toward cell-specific delivery. Effects of peptide antigenic determinant properties on adsorption equilibrium of anti-peptide antibodies. Kinetic and circular dichroism studies of enzymes adsorbed on ultrafine silica particles. Bioprocessing of Renewable Resources to Commodity Bioproducts, Wiley 2014 Chapter 8, Ethanol production from yeasts, Hasunuma, T. From transcriptome analysis, the Δ tubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Evaluation of cell surface-displayed protein stability against simulated gastric fluid. Journal of Geographic Information System, 3, 267-278. Ecological Research, 13, 355-365. Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction. Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment. Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. Preparation of thermo-sensitive magnetic microspheres and their application to bioprocesses. Enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. Production of glucoamylase by passively immobilized cells of a flocculent yeast, Saccharomyces diastaticus. In this study, we show the effects of YM on human colonic microbiota and its metabolites using an in vitro human faeces fermentation system. Biodiesel-fuel production in a packed-bed reactor using lipase-producing rhizopus oryzae cells immobilized within biomass support particles. Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum. Improvement of ethanol productivity during xylose and glucose co-fermentation by xylose-assimilating S. Overproduction and purification of SulA fusion protein in esherichia coli and its degradation by lon protease in vitro. Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and α-Amylase. Preparation of Thermus thermophilus holo-chaperonin-immobilized microspheres with high ability to facilitate protein refolding. Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles. Protein substrates and heat shock reduce the DNA-binding ability of esherichia coli lon protease. Specific Protein Delivery to Target Cells by Antibody-Displaying Bionanocapsules. Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase. Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch. Comparison of two forms of catalytic antibody displayed on yeast-cell surface. Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases. Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain. Biodiesel fuel production from plant oil catalyzed by Rhizoupusoryzae lipase in a water-containing system without an organic solvent. Biodiesel fuel production by transesterification of oils. Preparation of high-activity whole cell biocatalysts by permeabilization of recombinant yeasts with alcohol. Development of novel whole-cell immunoadsorbents by yeast surface display of IgG-binding domain. Breeding of industrial diploid yeast strain with chromosomal integration of multiple β-glucosidase genes. Int'l Workshop on Global Change and Terrestrial Environment in Monsoon Asia, 95-98. Environmental Research Center Papers, No. Nguen Viet Luong, Tateishi, R. The ratio was correlated with the pgi transcription level and the enzyme activity. Development and application of thermo-sensitive immunomicrospheres for antibody purification. Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose. Affinity selection of target cells from cell surface displayed libraries : a novel procedure using thermo-responsive magnetic nanoparticles. KRS101 on cellulosic biomass and the production of lipids containing high levels of docosahexaenoic acid. Advances in Space Research, 22 5 , 645-654. Over-production of various secretory-form proteins in Streptomyces lividans. Characterization of fractionated polyclonal antibodies for immunoaffinity chromatography. Wei Yang and Akihiko Kondoh 2020 : Evaluation of the Simard et al. Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production. Review Protein-protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling. Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts. Improvement of protein production in lactic acid bacteria using 5'-untranslated leader sequence of slpA from Lactobacillus acidophilus. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance. Dispersion of coagulated particles by contractile flow to orifice. Using Promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate. The resulting yeast genetic switches were readily integrated, in a plug-and-play manner, into an AND-gated carotenoid biosynthesis pathway. Effect of cultivation conditions on cell-surface display of Flo1 fusion protein using sake yeast. Mathematical Problems in Engineering, Vol. Hydrological Processes, 18, 3147-3158. Engineering strategy of yeast metabolism for higher alcohol production. Effects of adsorption conditions on kinetics of protein adsorption and conformational changes at ultrafine silica particles. We successfully improved productivity by redirecting pyruvate metabolism in the aerobic filamentous fungus Aspergillus oryzae via the deletion of two genes that encode pyruvate decarboxylase and mitochondrial pyruvate carriers. Efficient ethanol production from starch through development of novel flocculent yeast strains displaying glucoamylase and co-displaying or secreting α-amylase. Immunological agglutination behavior of latex particles with covalently immobilized antibodies. Hydrogeology Journal, 10, 587-599. Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast. Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture. Production of S-lactoylglutathione by high activity whole cell biocatalysts prepared by permeabilization of recombinant Saccharomyces cerevisiae with alcohols. Richa Bhattarai, Akihiko Kondoh 2017 :Risk Assessment of Land Subsidence in Kathmandu Valley, Nepal,Using Remote Sensing and GIS, Advances in Remote Sensing, 6, 132-146. Syndonia Bret-Harte, Donatella Zona, Walter Oechel, Akihiko Kondoh 2019 : A semi-analytical snow-free vegetation index for improving estimation of plant phenology in tundra and grassland ecosystems, Remote Sensing of Environment, 228, 31-44. Review Gene copy number and polyploidy on products formation in yeast. Atmospheric Environment, 45, 6682-6695. The in vitro model simulating human colonic microbiota revealed that E. Process engineering and optimization of glycerol separation in a packed-bed reactor for enzymatic biodiesel production. Role of N-terminal 28-amino-acid region of Rhizopus oryzae lipase in directing proteins to secretory pathway of Aspergillus oryzae. Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids. Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants. Review 2013 Enzymatic biodiesel production: An overview of potential feedstocks and process development. We found that changing dissolved oxygen and controlling pH were important factors for 1,3-butadiene production. Yi-Zhang Zhang, Xian-Fang Song, Akihiko Kondoh, Jun Xia, Chang-Yuan Tang 2011 : Behavior, mass inventories and modeling evaluation of xenobiotic endocrine-disrupting chemicals along an urban receiving wastewater river in Henan Province, China. Development of a novel aptamer-based sensing system using atomic force microscopy. Immobilized recombinant Aspergillus oryzae expressing heterologous lipase: an efficient whole-cell biocatalyst for enantioselective transesterification in non-aqueous Medium. Riset Geologi dan Pertambangan, Vol. Akihiko Kondoh and Akira Hama 2019 : "Chapter 17 Nuclear Disaster and Human Geoscience", Yukio Himiyama, Kenji Satake, Taikan Oki, eds. Hiroaki Abe, Changyuan Tang, Akihiko Kondoh 2014 : Effect of Urban Aquifer Exploitation on Subsurface Temperature and Water Quality. Overproduction and purification of lon protease from esherichia coli using maltose binding protein MBP fusion system. Review Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits. Journal of the Japan Society of Photogrammetry and Remote Sensing, 34, 2, 6-14. Selection of DNA aptamers using atomic force microscopy. Ethanol production from cellulosic materials using cellulase -expressing yeast. Review Applications of yeast cell-surface display in bio-refinery. Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst. Improvement of proteolytic resistance of immunoadsorbents by chemical modification with polyethylene glycol. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions. Development of a yeast protein fragment complementation assay PCA system using dihydrofolate reductase DHFR with specific additives. Moreover, a positive correlation was observed between the relative ratio with or without YM administration of B. Homo-D-lactic acid fermentation from arabinose by redirection of phosphoketolase pathway to pentose phosphate pathway in L-lactate dehydrogenase gene-deficient lactobacillus plantarum. Moreover, metabolically engineered strains of C. Direct ethanol production from barley β-glucan by sake yeast displaying Aspergillus oryzae β-glucosidase and endoglucanase. Double mutation of the PDC1 and ADH1 genes improves lactate production in the yeast Saccharomyces cerevisiae expressing the bovine lactate dehydrogenase gene. Wendisch, Christoph Wittmann Abstract The soil microbe Corynebacterium glutamicum is a leading workhorse in industrial biotechnology and has become famous for its power to synthetise amino acids and a range of bulk chemicals at high titre and yield. The specific delivery of proteins to human liver cells by engineered bio-nanocapsules. During dark periods with depressed CO 2 fixation, KOR1 shows rapid carbohydrate degradation together with increased lipid and carotenoid contents. The product portfolio of the microbe is continuously expanding. PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae. Of the Second Japan-UK Workshop on Implications of Recent Earthquakes on Seismic Risk, 77-84. Our study reveals the in vitro bifidogenic effect of DKT that likely contributes to its beneficial effects on the human colon. Effects of charged groups in haptens on adsorption equilibrium of hapten antibody. Enzyme-mediated site-specific antibody-protein modification using a ZZ domain as a linker. Secretory production system of bionanocapsules using a stably transfected insect cell line. Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids. Development of novel yeast cell surface display system for homo-oligomeric protein by coexpression of native and anchored subunits. Gγ recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions. Nobumitsu Tsunematsu, Hiroaki Kuze, Tomonori Sato, Masamitsu Hayasaki, Feifei Cui, Akihiko Kondoh 2011 : Potential impact of spatial patterns of future atmospheric warming on Asian dust emission. Pretreatment of immobilized Candida antarctica lipase for biodesel fuel production from plant oil. Youngjoo Kwak and A Kondoh 2010 A study on the assessment of multi-factors affecting urban floods using satellite image; a case study in Nakdong basin, S. Construction of Arginine-rich peptide displaying Bionanocapsules. To explore terminator sequences available to tune protein expression levels in P. Efficient production of L- + -lactic acid from raw starch by Streptococcus bovis 148. Lipase localization in rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production. Review 2014 Genetic engineering of bio-nanoparticles for drug delivery: a review. Direct Production of Cadaverine from Soluble Starch Using Corynebacterium glutamicum Coexpressing α-Amylase and Lysine Decarboxylase. Review 2013 Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing. Adsorption of g-globulin, a model protein for antibody, on colloidal particles. Atsushi Higuchi, Akihiko Kondoh, Shinkichi Kishi and Teruki Fukuzono 1996 : Relatioship between hydrological properties and multi-temporal AVHRR data in 1990, Huaihe river basin, China. Review 2012 Recent developments in yeast cell surface display toward extended applications in biotechnology. PCC 6803, probed by time-resolved fluorescence spectroscopy. Review 2012 Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering. Over-expression system for secretoryphospholipase D by streptomyceslividans. Efficient and direct glutathione production from raw starch using Saccharomyces cervisiae. Hydrological Research Letters, 14 1 , 56—61. Rapid and efficient selection of yeast displaying a target protein using thermo-responsive magnetic nanoparticles. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Market-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase Target-ACEmax showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. Here, we conducted a comprehensive metabolomic analysis to investigate the effect of the split ratio between glycolysis and the PPP on the intracellular concentration of amino acids and their derivatives in Corynebacterium glutamicum. Efficient secretory overexpression of Bacillus subtilis pectate lyase in Escherichia coli and single-step purification. Site-specific tetrameric streptavidin-protein conjugation using sortase A. Dilinur Aji, Akihiko Kondoh, Changyuan Tang 2008 :Analysis of hydrological changes of lakes and rivers in XinJiang using GIS techniques and remote sensing data. Hydrological Processes, 16, 2173-2187. Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilationg yeast strain. Komagataella phaffii has frequently been used as a platform for metabolic engineering but knowledge regarding P. Characterization of the immunointeraction and its application to bioaffinity separation. Effect of histidine residues in antigenic sites on pH dependence of immuno-adsorption equilibrium. Proceedings of the CEReS International Symposium on remote Sensing of the Atmosphere and validation of Satellite Data, CEReS, Chiba, Japan, pp. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the Δ tubB strain. In this study, we focused on controlling pyruvate metabolism in aerobic microorganisms for the biological production of various chemicals. Xylose isomerase from polycentric fungus Orpinomyces: gene sequencing, cloning, and expression in Saccharomyces cerevisiae for bioconversion of xylose to ethanol. Results We found that deletion of an α-tubulin tubB in T. Purification, characterization, and sequence determination of phospholipase D secreted by streptoverticillum cinnamoneum. Also, the tubulin-disrupted Δ tubB strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis α-amylase using cspB promoter and signal sequence. Advances in Geosciences, 4, 11-16. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Deletion of MCD4 involved in glycosylphosphatidylinositol GPI anchor synthesis leads to an increase in β-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae. Additionally, in pure cultures, B. Kuroda 2020 : Dialogue, radiation measurements and other collaborative practices by experts and residents in the former evacuation areas of Fukushima: A case study in Yamakiya District, Kawamata Town, Radioprotection, 55 3 , 215—224. An Improved Method of Lipase Preparation Incorporating Both Solvent Treatment and Immobilization onto Matrix. Yeast cell surface display-application of molecular display. Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling. Song 2004 : Measurement and analysis of the redistribution of soil moisture and solutes in a maize field in the lower reaches of the Yellow River. Next-generation sequencing of bacterial 16S rRNA gene revealed a significant increase in the relative abundance of bacteria related to the Bifidobacterium genus in the model after incubation with DKT. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato Solanum lycopersicum L. This study constructed a novel artificial metabolic pathway for 1,3-butadiene production from glucose in Escherichia coli by combining the cis,cis-muconic acid ccMA -producing pathway and tailored ferulic acid decarboxylase mutants. Comparison of conformational changes of hemoglobin and myoglobin upon adsorption on various inorganic ultrafine particles. Novel strategy for yeast construction using δ-integration and cell fusion to efficiently produce ethanol from raw starch. In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. Preparation of immobilized enzyme with high activity using affinity tag based on protein A and G. Alcoholic fermentation of xylose and mixed sugars using recombinant Saccharomyces cerevisiae engineered for xylose utilization. Spectral changes of lysozyme adsorbed on ultrafine silica particles. The Future of Cranes and Wetlands, 61-71. Improvement of isoflavone aglycones production using β-glucosidase secretory produced in recombinant Aspergillus oryzae. Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from candida antarctica. Construction of ethanol-tolerant yeast strains with combinatorial library-selected peptides. Enhancement of β-glucosidase activity on the cell-surface of sake yeast by disruption of SED1. This study aimed to determine the effect of E. Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production. Physicochemical and immunological characterization of hepatitis B virus envelope particles exclusively consisting of the entire L Pre-S1+Pre-S2+S protein. Display of α-amylase on the surface of Lactobacillus casei cells by use of the PgsA anchor protein, and production of lactic acid from starch. DKT was added to the in vitro microbiota model culture at a concentration of 0. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. High-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme DBE. Use of a substrate as an encapsulated marker for liposome immunoassay. Development of efficient protein refolding systems using chaperonins. Efficient immobilization of protein on monodispersed colloidal silica particles modified by copolymer of maleic anhydride and styrene or methyl methacrylate. Construction of engineered yeast with the ability of binding to cellulose. These results suggested that the Δ tubB strain was released from CCR. Isolation of novel catalytic antibody clones from combinatorial library displayed on yeast-cell surface. Efficient production of ethanol from raw starch by a mated diploid Saccharomyces cerevisiae with integrated α-amylase and glucoamylase genes. Adsorption equilibrium in immuno-affinity chromatography with polyclonal and monoclonal antibodies. Affinity purification of antibodies using immunomicrospheres. Construction of whole-cell biocatalyst for xylan degradation through cell-surface xylanase display in Saccharomyces cerevisiae. Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression. Long anchor using Flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates. Review 2012 Enzyme-mediated methodologies for protein modification and bioconjugate synthesis. Because selection threshold of both ON- and OFF-state selection can be flexibly tuned, the desired selection conditions can be rapidly pinned down for individual directed evolution experiments without a prior knowledge either on the library population. Herein, we highlight recent advances in engineering of the microbe into novel cell factories that overproduce these precious molecules from pioneering proofs-of-concept up to industrial productivity. Hiroaki Abe, Changyuan Tang, Nozomu Takeuchi, Akihiko Kondoh 2018 : Influence of Seasonal Pumping on Groundwater Sources and Flow System, Nagaoka Plain, Japan. System using tandem repeats of the cA peptidoglycan-binding domain from Lactococcus lactis for display of both N- and C-terminal fusions on cell surfaces of lactic acid bacteria. Fatty acid production from butter using novel cutinase-displaying yeast. We aimed to determine how DKT affects human colonic microbiota. Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. Bioconversion using immobilized recombinant flocculent yeast cells carrying a fused enzyme gene in an "intelligent" bioreactor. Phospholipase D production using immobilized cells of streptoverticillium cinnamoneum. Journal of Forest research, 3, 111-117. Engineered bio-nanocapsules, the selective vector for drug delivery system. Homo-D-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum. Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier. Immunological agglutination kinetics of latex particles with covalently immobilized antigens. Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. Development of an enzyme activity screening system for β-glucosidase-displaying yeasts using calcium alginate micro-beads and flow sorting. Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation. Methods in molecular biology, Vol. PCC 6803 for the production of lactic acid. Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein. Application of remote sensing and GIS for anthropogenic vegetation monitoring. Conformational changes in protein molecules upon adsorption on ultrafine particles. Fresh Perspectives on Hydrology and Water Resources in Southeast Asia and the Pacific, IHP-V Technical Document in Hydrology No. Review 2012 Bioconversion of lignocellulose-derived sugars to ethanol by engineered Saccharomyces cerevisiae. The non-conventional yeast Pichia pastoris syn. Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast. Display of a functional hetero-oligomeric catalytic antibody on the yeast cell surface. Reaction properties of immobilized catalytic antibodies that deprotect acylated Carbohydrates. In addition, YM administration increased the production of acetate, propionate, and total short-chain fatty acids. Biofunctional TiO2 nanoparticle-mediated photokilling of cancer cells using UV irradiation. Jianyao Chen, Changyuan Tang, Yasuo Sakura, Akihiko Kondoh, Jingjie Yu, Jun Shimada and Tadashi Tanaka 2004 :Spatial geochemical and isotopic characteristics associated with groundwater flow in the North China Plain. Concentrations of threonine and lysine-derivative 1,5-diaminopentane increased with an increase of the split ratio into the PPP. However, microorganisms cannot directly produce 1,3-butadiene using glucose as a renewable carbon source via biological processes. Preparation of yeast strains displaying IgG binding domain ZZ and enhanced green fluorescent protein for novel antigen detection systems. Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation. Journal of the Japan Society of Photogrammetry and Remote Sensing, 39 4 , 64-73. JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Circular dichroism studies on conformational changes in protein molecules upon adsorption on ultrafine polystyrene particles. Display of active enzymes on the cell surface of escherichia coli using PgsA anchor protein and their application to bioconversion. Bacterial 16S rRNA gene sequence analysis showed that YM administration did not change the microbial diversity or composition. Determination and analysis of the starch binding domain of Streptococcus bovis 148 raw-starch-hydrolysing α-amylase. Collectively, these findings indicate that E.。

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